The role of zinc in the adaptation of diatoms to conditions of polar oceans (MOCK_UENV25ARIES )
Key Details
- Application Deadline
- 8 January 2025 (midnight UK time)
- Location
- UEA
- Funding type
- Competition Funded Project (UK and International)
- Start date
- 1 October 2025
- Mode of study
- Full or part time
- Programme type
- PhD
Project description
Primary supervisor - Professor Thomas Mock
Scientific background
Diatoms are the main primary producers in polar oceans, where photosynthesis is largely limited by seasonal fluctuation in light, temperature and the extent of sea ice. Additionally, essential trace metals such as iron and zinc play an important role in controlling the biomass of polar primary producers. Polar diatoms appear to have a particularly high demand for zinc, thereby largely determining zinc distribution throughout the global ocean. The reason for the enhanced requirement of zinc in polar diatoms remains enigmatic. However, the first genome sequences from a polar diatom and other cold-adapted algae revealed adaptive expansions of gene families containing zinc-binding domains. The elevated concentrations of zinc in polar oceans may thus have aided the expansion of these zinc-binding domains. As specific gene families involved in photosynthesis and carbon fixation were both co-expanded and co-expressed, it suggests that zinc plays an important role in supporting photosynthetic growth in polar phytoplankton.
Research methodology
The main aim of this project is to produce the first molecular genetics and biochemical data on the role of zinc in the physiological adaptation of cold-adapted diatoms. You will work in the laboratory with a cold-adapted model diatom and will apply the latest reverse genetics tools (e.g. CRISPR-Cas) in combination with sequencing (RNA/DNA) and photosynthesis measurements (e.g. carbon acquisition, quantum yield) to characterise to role of conserved low-temperature inducible regulatory genes with zinc-binding domains (e.g. zf-MYND) that are co-regulated with photosynthesis genes. A combination of these experimental approaches together with an evolutionary analysis will provide first insights into the role of zinc-binding domains in supporting photosynthesis in polar marine microalgae.
Training
You will gain skills in the latest reverse-genetics tools such as CRISPR-Cas and sequence analyses, algal cultivation, photosynthesis measurements, protein biochemistry, bioinformatics and evolutionary biology.
Person specification
A degree in Biological Science or equivalent. We are looking for an enthusiastic student who is excited about applying diverse techniques from the field of molecular microbiology to understand the adaptation and evolution of microalgae in polar oceans.
Entry requirements
The minimum entry requirement is 2:1 in a Bachelor’s degree in Biological Sciences or equivalent.
Funding
This project has been shortlisted for funding under the ARIES BBSRC-NERC Doctoral Landscape Award (DLA) scheme. Successful candidates who meet UKRI's eligibility criteria will be awarded a fully-funded ARIES studentship of fees, maintenance stipend (19,237 p.a for 2024/25) and research costs.
A limited number of ARIES studentships are available to International applicants. Please note however that ARIES funding does not cover additional costs associated with relocation to, and living in, the UK.
ARIES is committed to equality, diversity, widening participation and inclusion in all areas of its operation. We encourage applications from all sections of the community regardless of gender, ethnicity, disability, age, sexual orientation, and transgender status. Projects have been developed with consideration of a safe, inclusive, and appropriate research and fieldwork environment. Academic qualifications are considered alongside non-academic experience, with equal weighting given to experience and potential.
For further information, please visit www.aries-dtp.ac.uk
References
Ye et al. (2022) The role of zinc in the adaptive evolution of polar phytoplankton. Nature Ecology and Evolution (https://www.nature.com/articles/s41559-022-01750-x)
Clark et al. (2023) Multi-omics for studying and understanding polar life. Nature Communications (https://www.nature.com/articles/s41467-023-43209-y)
Strauss et al. (2023) Plastid-localized xanthorhodopsin increases diatom biomass and ecosystems productivity in iron-limited surface oceans. Nature Microbiology (https://www.nature.com/articles/s41564-023-01498-5)
Falciatore & Mock (Eds.) (2022) The Molecular Life of Diatoms, pp 808, Springer International Publishing (https://link.springer.com/book/9783030924980)
Mock et al. (2017) Evolutionary genomics of the cold-adapted diatom Fragilariopsis cylindrus. Nature (DOI: 10.1038/nature20803)